5T4/TPBG Protein, Mouse, Recombinant (His Tag)
Product Specifications
Background
Trophoblast glycoprotein (TPBG), also known as 5T4, is the therapeutic target of several anticancer agents currently in clinical development, largely due to its high expression in tumors and low expression in normal adult tissues.
Synonyms
5T4 Protein, Mouse; AW495680 Protein, Mouse; WAIF1 Protein, Mouse
Gene Name
Trophoblast glycoprotein
Expression System
HEK293 Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant mouse TPBG consists of 341 amino acids and predicts a molecular mass of 37.2 kDa. It migrates as an approximately 53.8 KDa band in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PM53120M2.pdf
Symbol
TPBG
Species
Mouse
Protein ID
NP_001158264.1
Overview
A DNA sequence encoding the mouse TPBG (NP_001158264.1) (Met1-Ser361) was expressed with a polyhistidine tag at the C-terminus.
AA Sequence
Met1-Ser361
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_001158264.1
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