CD7 Protein, Mouse, Recombinant (His Tag)
Product Specifications
Background
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD7 is a transmembrane protein that is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell / B-cell interaction during early lymphoid development.
Overview
A DNA sequence encoding the mouse CD7 (NP_033984.1) extracellular domain (Met 1-Pro 150) was fused with a polyhistidine tag at the C-terminus.
Gene Name
CD7 molecule
Expression System
HEK293 Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 94 % as determined by SDS-PAGE.
Activity
Measured by its ability to bind biotinylated recombinant human SECTM1 in a functional ELISA.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
CD Molecule proteins, Diagnostic proteins, Enzyme Proteins
Molecular Weight
The secreted recombinant mouse CD7 comprises 138 amino acids and has a predicted molecular mass of 15.7 kDa. As a result of glycosylation, the apparent molecular mass of rmCD7 is approximately 20-30 kDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
CD7
Species
Mouse
Protein ID
NP_033984.1
AA Sequence
Met1-Pro150
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_033984.1
Available Sizes
Frequently Asked Questions
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