BACE2 Protein, Mouse, Recombinant (His Tag)
Product Specifications
Background
BACE2, also known as beta secretase 2, belongs to the peptidase A1 family. It is a protease known to be an important enzyme involved in the cellular pathways. BACE2 has been shown to interact with GGA1 and GGA2. It is the major β-secretase in vivo. BACE2 is located on chromosome 21 and may play a role in alzheimer's disease pathogenesis in down syndrome (DS) . Overexpression of BACE2 by lentivirus markedly reduced amyloid β protein production in primary neurons. Despite an extra copy of the BACE2 gene in DS and the increase of its transcription, BACE2 protein levels are unchanged.
Synonyms
1110059C24Rik Protein, Mouse; AEPLC Protein, Mouse; AI850424 Protein, Mouse; ALP56 Protein, Mouse; ARP1 Protein, Mouse; ASP21 Protein, Mouse; BAE2 Protein, Mouse; CDA13 Protein, Mouse; CEAP1 Protein, Mouse; DRAP Protein, Mouse
Gene Name
Beta-site APP-cleaving enzyme 2
UniProt
Q9JL18
Expression System
HEK293 Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog # ES010) .The specific activity is >50 pmoles/min/μg.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Protease proteins, Enzyme Proteins
Molecular Weight
The recombinant mouse BACE2 comprises 454 amino acids and has a predicted molecular mass of 49.2 kDa. The apparent molecular mass of the protein is approximately 55 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PM53055M2.pdf
Symbol
BACE2
Species
Mouse
Overview
A DNA sequence encoding the mouse BACE2 (Q9JL18) (Met1-Pro462) was expressed with a C-terminal polyhistidine tag.
Uniprot URL
https://www.uniprot.org/uniprot/Q9JL18
AA Sequence
Met1-Pro462
Available Sizes
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