KGF/FGF-7 Protein, Mouse, Recombinant (His Tag)

Product Specifications

Background

Fibroblast growth factor 7 (FGF7) is a member of the fibroblast growth factor (FGF) family of proteins. FGF7 plays an important role in regulating the proliferation, migration, and differentiation of cells. FGF7 is of stromal origin and produces a paracrine effect on epithelial cells. FGF7 is a mesenchyme-specific heparin-binding growth factor that binds FGF receptor 2 (FGFR2) to regulate numerous cellular and physiological processes. FGF7/FGFR2 promotes invasion and migration in human gastric cancer. FGF7 is specifically utilized as a paracrine factor during the process of differentiation of the epidermal layers in the regenerating scales and in particular for beta-cells differentiation. FGF7 over expression is associated with advanced clinical features in patients with upper tract and bladder urothelial carcinoma, justifying its potential prognostic value for urothelial carcinoma.

Overview

A DNA sequence encoding the mouse FGF7 (NP_032034.1) (Cys32-Thr194) was expressed with a polyhistidine tag at the N-terminus.

Synonyms

Kgf Protein, Mouse

Gene Name

Fibroblast growth factor 7

Expression System

E. coli

Tag

N-His

Endotoxin

Please contact us for more information.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Measured in a cell proliferation assay using 4MBr 5 rhesus monkey epithelial cells. The EDfor this effect is 15-90 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile 10 mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl, 140 mM NaCl, 15 % glycerol. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cytokine Proteins, Growth Factor proteins, Cell Therapy proteins, Biological Target proteins

Molecular Weight

The recombinant mouse FGF7 consists of 181 amino acids and predicts a molecular mass of 21 kDa. It migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.