FGF2 Protein, Mouse, Recombinant (His Tag)

Product Specifications

Background

Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI) . bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.

Overview

A DNA sequence encoding the mouse Fgf2 (NP_032032.1) (Ala11-Ser154) was expressed with a polyhistidine tag at the N-terminus.

Synonyms

BFGF Protein, Mouse; Fgf-2 Protein, Mouse; Fgfb Protein, Mouse

Gene Name

Fibroblast growth factor 2 (basic)

Expression System

E. coli

Tag

N-His

Field of Research

Cancer Drug Targets

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts.The EDfor this effect is typically 0.1-0.6 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cytokine Proteins, Growth Factor proteins, Biological Target proteins, Diagnostic proteins, Cell Therapy proteins, Biomarker Proteins

Molecular Weight

The recombinant mouse Fgf2 consists 162 amino acids and predicts a molecular mass of 18.5 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.