IFNA14 Protein, Mouse, Recombinant (hFc Tag)

Product Specifications

Overview

A DNA sequence encoding the secreted form of mouse IFNA14 (NP_996858.1) (Cys 24-Lys 189) was fused with the Fc region of human IgG1 at the N-terminus.

Synonyms

OTTMUSG00000007662 Protein, Mouse

Gene Name

Interferon, alpha 14

Expression System

HEK293 Cells

Tag

N-hFc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by SDS-PAGE.

Activity

Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV) .The EDfor this effect is typically 10-100 pg/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cytokine Proteins, Growth Factor proteins, Biological Target proteins

Molecular Weight

The recombinant mouse IFNA14/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 426 amino acids and has a predicted molecular mass of 47.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm IFNA14/Fc monomer is approximately 50 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.