PLGF/PGF Protein, Mouse, Recombinant (hFc Tag)
Product Specifications
Synonyms
AI854365 Protein, Mouse; PIGF Protein, Mouse; Plgf Protein, Mouse
Gene Name
Placental growth factor
UniProt
P49764
Expression System
HEK293 Cells
Tag
C-hFc
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 76 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Cytokine Proteins, Growth Factor proteins, Biological Target proteins
Molecular Weight
The recombinant mouse PGF/Fc is a disulfide-linked homodimer. The reduced monomer comprises 381 amino acids and has a calculated molecular mass of 42.9 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 52 kDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PM52944M4.pdf
Symbol
PGF
Species
Mouse
Overview
A DNA sequence encoding the mouse PGF (P49764) (Met 1-Pro 158) was fused with the Fc region of human IgG1 at the C-terminus.
Uniprot URL
https://www.uniprot.org/uniprot/P49764
AA Sequence
Met1-Pro158
Available Sizes
Curated Selection
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