CD200R4 Protein, Mouse, Recombinant (His Tag)

Cell surface glycoprotein CD2 receptor 4, also known as Cell surface glycoprotein OX2 receptor 4, CD2 cell surface glycoprotein receptor-like 4, CD2RLa, and CD2R4, is a single-pass type I membrane protein which belongs to theCD2R family. CD2 (OX2) is a cell surface glycoprotein that interacts with a structurally related receptor (CD2R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. In mouse there are up to five genes related to CD2R with conflicting data as to whether they bind CD2. CD2R4 contains oneIg-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. CD2R4 is highly expressed in monocytes, NK cells and a subset of NKT cells. It is weakly expressed in granulocytes and B cells (at protein level) . CD2R4 is also expressed in brain, lung, testis, thymus, intestine and uterus. and in bone marrow derived-macrophage and dendritic cells and mast cells. CD2R4 is involved in the recruitment or surface expression of the TYROBP receptor.

A DNA sequence encoding the extracellular domain of mouse CD200R4 (NP_997127.1) (Met 1-Thr 241) was expressed, fused with a polyhistidine tag at the C-terminus.

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The secreted recombinant mouse CD200R4 comprises 227 amino acids and has a predicted molecular mass of 25 kDa. As a result of glycosylation, the apparent molecular mass of rm CD200R4 is approximately 50-60 kDa in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.