Kallikrein 1 Protein, Mouse, Recombinant (His Tag)

Product Specifications

Background

KLK1 (Kallikrein 1) is a Protein Coding gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. Human tissue kallikrein (KLK1) is a serine protease, a component of the KKS that has been demonstrated to exert pleiotropic beneficial effects in protection from tissue injury through its anti-inflammatory, antiapoptotic, antifibrotic, and antioxidative actions. Polymorphism of the human tissue kallikrein 1 (KLK1) A1789G gene is associated with susceptibility to hypertension. Diseases associated with KLK1 include Kallikrein, Decreased Urinary Activity Of and Hereditary Angioedema.

Overview

A DNA sequence encoding the mouse KLK1 (P15947) (Met1-Asp261) was expressed with a C-terminal polyhistidine tag.

Synonyms

0610007D04Rik Protein, Mouse; Kal Protein, Mouse; Klk1b6 Protein, Mouse; Klk6 Protein, Mouse; mGk-6 Protein, Mouse

Gene Name

Kallikrein 1

UniProt

P15947

Expression System

HEK293 Cells

Tag

C-His

Field of Research

Serine Proteases and Regulators

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC) .The specific activity is >6, 000 pmol/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Form

Lyophilized

Buffer

Lyophilized from sterile 25 mM Tris, 5 mM CaCl2, 0.15 M NaCl, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins, Protease proteins

Molecular Weight

The recombinant mouse KLK1 comprises 254 amino acids and has a predicted molecular mass of 28.3 kDa. The apparent molecular mass of the protein is approximately 36 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.