Serum Amyloid P Protein, Mouse, Recombinant (His Tag)

Product Specifications

Background

Serum amyloid P component (SAP) is the identical serum form of amyloid P component (AP), a highly preserved plasma protein named for its ubiquitous presence in amyloid deposits. As a normal plasma protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". Serum amyloid P component represents another member of the pentraxin family, a highly conserved group of molecules that may play a role in innate immunity. SAP is a key negative regulator for innate immune responses to DNA and may be partly responsible for the insufficient immune responses after DNA vaccinations in humans. SAP suppression may be a novel strategy for improving efficacy of human DNA vaccines and requires further clinical investigations.

Overview

A DNA sequence encoding the mouse APCS (NP_035448.2) (Met 1-Asp 224) precursor was expressed with a C-terminal polyhistidine tag.

Synonyms

Sap Protein, Mouse

Gene Name

Amyloid P component, serum

Expression System

HEK293 Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

1. Measured by its binding ability in a functional ELISA. Immobilized mouse APCS at 10 μg/ml (100 μl/well) can bind biotinylated human Fibronectin Fragment 2 with a linear range of 0.625-5 μg/ml. 2. Measured by its ability to bind mouse CD64-AVI in a functional ELISA.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The secreted recombinant mouse APCS consists of 215 amino acids and has a calculated molecular mass of 25.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 28 kDa protein in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.