CD32B/Fcgr2b Protein, Mouse, Recombinant (His Tag)
Product Specifications
Synonyms
AI528646 Protein, Mouse; CD32 Protein, Mouse; F630109E10Rik Protein, Mouse; Fcgr2 Protein, Mouse; Fcgr2a Protein, Mouse; FcgRII Protein, Mouse; Fcr-2 Protein, Mouse; Fcr-3 Protein, Mouse; fcRII Protein, Mouse; Fc[g]RII Protein, Mouse; Ly-17 Protein, Mouse; Ly-m20 Protein, Mouse; LyM-1 Protein, Mouse
Gene Name
Fc fragment of IgG, low affinity IIb, receptor (CD32)
Expression System
HEK293 Cells
Tag
C-His
Field of Research
Fc Receptors ITIM/ITAM Immunoreceptors and Related Molecules
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 98 % as determined by SDS-PAGE.
Activity
Measured by its binding ability in a functional ELISA.Immobilized mouse FCGR2B-His (CD32) (Cat:PM52891M2) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The ECof biotinylated human IgG1 is 0.13-0.29 μg/ml.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Fc receptor proteins, Immunotherapy proteins, CD Molecule proteins, Receptor Proteins
Molecular Weight
The secreted recombinant mouse FCGR2B consists of 189 amino acids and has a predicted molecular mass of 21.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR2B is approximately 34 kDa due to glycosylation.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PM52891M2.pdf
Symbol
FCGR2B
Species
Mouse
Protein ID
NP_001070657.1
Overview
A DNA sequence encoding the extracellular domain (Met 1-Arg 217) of mouse FCGR2B (NP_001070657.1) precursor was expressed with a polyhistidine tag at the C-terminus.
AA Sequence
Met1-Arg217
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_001070657.1
Available Sizes
Curated Selection
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