TNFR-1/CD120a Protein, Mouse, Recombinant (hFc Tag)

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD) . CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

CD120a Protein, Mouse; FPF Protein, Mouse; p55 Protein, Mouse; p55-R Protein, Mouse; TNF-alphaR1 Protein, Mouse; TNF-R Protein, Mouse; TNF-R-I Protein, Mouse; TNF-R1 Protein, Mouse; TNF-R55 Protein, Mouse; TNFalpha-R1 Protein, Mouse; TNFAR Protein, Mouse; Tnfr-2 Protein, Mouse; Tnfr1 Protein, Mouse; TNFR60 Protein, Mouse; TNFRI Protein, Mouse; TNFRp55 Protein, Mouse

1. Measured by its binding ability in a functional ELISA. Immobilized Mouse TNF-alpha (Cat:PM53227E0) at 2 μg/ml (100 μl/well) can bind Mouse TNFRSF1AhFc (Cat:PM52815M4), the ECof Mouse TNFRSF1AhFc is 1.5-9.0 ng/mL. 2. Measured by its ability to inhibit the TNFα-mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The EDfor this effect is typically 15-65 ng/mL in the presence of 0.1 ng/mL of recombinant mouse TNFα.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The secreted recombinant mouse TNFRSF1A/Fc is a disulfide-linked homodimer. The reduced monomer consists of 424 amino acids and has a predicted molecular mass of 47.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm TNFRSF1A/Fc monomer is approximately 55 kDa due to glycosylation.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the mouse TNFRSF1A (NP_ 035739.2) extracellular domain (Met 1-Ala 212) was fused with the Fc region of human IgG1 at the C-terminus.