Ephrin B2 Protein, Mouse, Recombinant (His Tag)

Product Specifications

Background

EphrinB2 also known as EFNB2 is a member of the ephrin family. EphrinB2 is involved in establishing arterial versus venous identity and perhaps in anastamosing arterial and venous vessels at their junctions. The transmembrane-associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB2 expression progressively extends from the arterial endothelium to surrounding smooth muscle cells and to pericytes, suggesting that ephrin-B2 may play an important role during formation of the arterial muscle wall.

Overview

A DNA sequence encoding the mouse EFNB2 (NP_034241.2) extracellular domain (Met 1-Ala 232) was fused with a polyhistidine tag at the C-terminus.

Synonyms

ELF-2 Protein, Mouse; Epl5 Protein, Mouse; Eplg5 Protein, Mouse; Htk-L Protein, Mouse; LERK-5 Protein, Mouse; Lerk5 Protein, Mouse; NLERK-1 Protein, Mouse

Gene Name

Ephrin-B2

Expression System

HEK293 Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Immobilized Ephrin B2 Protein, Mouse, Recombinant (His Tag) (Cat:PM52802M2) at 2 μg/mL (100 μL/well) can bind EphB4 Protein, Mouse, Recombinant (hFc Tag) (Cat:PM53150M4), the ECis 3-18 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Growth Factor proteins, Cytokine Proteins, Biological Target proteins

Molecular Weight

The secreted recombinant mouse EFNB2 comprises 215 amino acids and has a predicted molecular mass of 23.5 kDa. As a result of glycosylation, the apparent molecular mass of rmEFNB2 is approximately 30-40 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.