Apolipoprotein A-I/APOA1 Protein, Mouse, Recombinant (hFc Tag)

Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease.

1-Sep Protein, Mouse; 2-Sep Protein, Mouse; 42248 Protein, Mouse; 42249 Protein, Mouse; Alp-1 Protein, Mouse; apo-AI Protein, Mouse; Apoa-1 Protein, Mouse; apoA-I Protein, Mouse; Brp-14 Protein, Mouse; Ltw-1 Protein, Mouse; Lvtw-1 Protein, Mouse

1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse ApoAI at 10 μg/mL (100 μl/well) can bind biotinylated human SCARB1, The ECof biotinylated human SCARB1 is 0.27 μg/mL.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The secreted recombinant mouse APOA1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 487 amino acids and has a calculated molecular mass of 55.8 kDa. As a result of glycosylation, the apparent molecular mass of rmAPOA1/Fc monomer is approximately 60 kDa in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.