CRELD2 Protein, Mouse, Recombinant (hFc Tag)
Product Specifications
Overview
A DNA sequence encoding the mouse Creld2 (NP_083996.1) (Met1-Leu350) was expressed with the Fc region of human IgG1 at the C-terminus.
Synonyms
5730592L21Rik Protein, Mouse; C85758 Protein, Mouse
Gene Name
Cysteine-rich with EGF-like domains 2
Expression System
HEK293 Cells
Tag
C-hFc
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE.
Activity
Measured by the ability of the immobilized protein to support the adhesion of ATDC5 mouse chondrogenic cells.When 5 x 10^4 cells/well are added to mCRELD2-coated plates (0.625 μg/mL, 100 μL/well), approximately 30% cells will adhere after 60 minutes at 37°C.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant mouse Creld2 consists 566 amino acids and predicts a molecular mass of 62.8 kDa.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
CRELD2
Species
Mouse
Protein ID
NP_083996.1
AA Sequence
Met1-Leu350
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_083996.1
Frequently Asked Questions
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