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CRELD2 Protein, Mouse, Recombinant (hFc Tag)

Product Specifications

Overview

A DNA sequence encoding the mouse Creld2 (NP_083996.1) (Met1-Leu350) was expressed with the Fc region of human IgG1 at the C-terminus.

Synonyms

5730592L21Rik Protein, Mouse; C85758 Protein, Mouse

Gene Name

Cysteine-rich with EGF-like domains 2

Expression System

HEK293 Cells

Tag

C-hFc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Measured by the ability of the immobilized protein to support the adhesion of ATDC5 mouse chondrogenic cells.When 5 x 10^4 cells/well are added to mCRELD2-coated plates (0.625 μg/mL, 100 μL/well), approximately 30% cells will adhere after 60 minutes at 37°C.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant mouse Creld2 consists 566 amino acids and predicts a molecular mass of 62.8 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

CRELD2

Species

Mouse

Protein ID

NP_083996.1

AA Sequence

Met1-Leu350

Protein ID Link

https://www.ncbi.nlm.nih.gov/protein/NP_083996.1

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