REG2 Protein, Mouse, Recombinant (hFc Tag)

Regenerating islet-derived proteins (Regs) comprise the superfamily of C-type lectin proteins encoded by Reg1, Reg2, Reg3a, Reg3b, Reg3g, Reg3d, and Reg4. The Reg family proteins are expressed in various tissues and have pleiotropic functions. Reg3b and Reg3g encode murine Reg3β and Reg3γ, respectively, and are murine homolog of human REG3A. Both proteins are highly expressed in the small intestine of adult mice at both mRNA and protein levels, but their expression is very low in the colon. Intriguingly, expression of Reg3β and Reg3γ is not detectable in the embryonic mouse intestine but gradually increases along with colonization of the commensal bacteria after birth.

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant mouse REG2 comprises 389 amino acids and has a predicted molecular mass of 43.6 kDa. The apparent molecular mass of the protein is approximately 45.4 kDa in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.