HAI-1 Protein, Mouse, Recombinant (hFc Tag)

Product Specifications

Overview

A DNA sequence encoding the mouse Spint1 (NP_058603.2) (Met1-Glu443) was expressed with the Fc region of human IgG1 at the C-terminus.

Synonyms

HAI-1 Protein, Mouse; Hai1 Protein, Mouse

Gene Name

Serine peptidase inhibitor, Kunitz type 1

Expression System

HEK293 Cells

Tag

C-hFc

Field of Research

Serine Proteases and Regulators

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys (DNP) -NH2.The IC50 value is < 1.5 nM.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant mouse Spint1 consists 652 amino acids and predicts a molecular mass of 73.2 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.