HAI-1 Protein, Mouse, Recombinant (hFc Tag)
Product Specifications
Overview
A DNA sequence encoding the mouse Spint1 (NP_058603.2) (Met1-Glu443) was expressed with the Fc region of human IgG1 at the C-terminus.
Synonyms
HAI-1 Protein, Mouse; Hai1 Protein, Mouse
Gene Name
Serine peptidase inhibitor, Kunitz type 1
Expression System
HEK293 Cells
Tag
C-hFc
Field of Research
Serine Proteases and Regulators
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys (DNP) -NH2.The IC50 value is < 1.5 nM.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant mouse Spint1 consists 652 amino acids and predicts a molecular mass of 73.2 kDa.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
SPINT1
Species
Mouse
Protein ID
NP_058603.2
AA Sequence
Met1-Glu443
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_058603.2
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