FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His & AVI Tag), Biotinylated

Product Specifications

Overview

A DNA sequence encoding the mouse FCGRT (NP_034319.2) (Met 1-Ser 297) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse B2M (P01887,105Ala/Asp) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Synonyms

FcRn Protein, Mouse

Gene Name

Fc fragment of IgG, receptor, transporter, alpha

UniProt

P01887

Expression System

HEK293 Cells

Tag

C-AVI & His

Field of Research

Fc Receptors

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its ability to bind recombinant rhFc (CHO) (Cat:PH52028M0) in a functional ELISA.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Receptor Proteins, Fc receptor proteins, Biotinylated proteins

Molecular Weight

The recombinant mouse FCGRT / B2M consists of 401 (302 + 99) amino acids and predicts a molecular mass of 46 (34.3 + 11.7) kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.