SPOCK3 Protein, Mouse, Recombinant (His Tag)

Product Specifications

Overview

A DNA sequence encoding the mouse Spock3 (NP_076178.1) (Met1-Ile436) was expressed with a polyhistidine tag at the C-terminus.

Synonyms

2900045C01Rik Protein, Mouse; AI428471 Protein, Mouse; mKIAA4039 Protein, Mouse

Gene Name

Sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3

Expression System

Baculovirus-Insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-Leu-Arg-AMC.The IC50 value is < 15 nM.

Form

Lyophilized

Buffer

Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins, Protease proteins

Molecular Weight

The recombinant mouse Spock3 consists 425 amino acids and predicts a molecular mass of 48.3 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.