CD48 Protein, Mouse, Recombinant (His Tag), Biotinylated

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.

A DNA sequence encoding the mouse CD48 (NP_031675.1) without the propeptide, corresponding to the amino acids (Met1-Arg216) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.

AI449234 Protein, Mouse; AW610730 Protein, Mouse; Bcm-1 Protein, Mouse; BCM1 Protein, Mouse; BLAST Protein, Mouse; BLAST-1 Protein, Mouse; BLAST1 Protein, Mouse; MEM-102 Protein, Mouse; Sgp-60 Protein, Mouse; SLAMF2 Protein, Mouse

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The secreted recombinant mouse CD48 consists of 205 amino acids and has a calculated molecular mass of 23.7 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 45.5 kDa in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.