FCGR4 Protein, Mouse, Recombinant (His Tag)

Product Specifications

Overview

The amino acid sequence corronding to Met 1-Gln 203 of mouse FCGR4 (NP_653142.2) was fused with a polyhistidine tag at the C-terminus.

Synonyms

4833442P21Rik Protein, Mouse; CD16-2 Protein, Mouse; FcgammaRIV Protein, Mouse; Fcgr3a Protein, Mouse; FcgRIV Protein, Mouse; Fcrl3 Protein, Mouse

Gene Name

Fc receptor, IgG, low affinity IV

Expression System

HEK293 Cells

Tag

C-His

Field of Research

Fc Receptors

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA.Immobilized mouse FCGR4 (Cat:PM52634M2) at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc) (Cat:PH52028M0) . The ECof human IgG1 (Fc) (Cat:PH52028M0) is 0.11-0.25 μg/ml.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Receptor Proteins, Fc receptor proteins

Molecular Weight

The recombinant mouse FCGR4 consists of 194 amino acids and has a predicted molecular mass of 22.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 25-35 kDa due to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.