CD27 Protein, Human, Recombinant (His Tag)

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

Measured by its binding ability in a functional ELISA.Immobilized human CD27-his (Cat:PH52446I2) at 10 μg/mL (100 μl/well) can bind biotinylated human CD70-Fc (Cat:PH50351M3), The ECof biotinylated human CD70-Fc (Cat:PH50351M3) is 57-85 ng/mL.

Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

TNF Superfamily proteins, CD Molecule proteins, Receptor Proteins, Cancer proteins, Immunotherapy proteins, Cytokine Proteins, Immune Checkpoint Proteins, Biological Target proteins, Diagnostic proteins

The secreted recombinant human CD27 consists of 183 amino acids and predicts a molecular mass of 20.7 KDa. The apparent molecular mass of the protein is approximately 27 KDa in SDS-PAGE under reducing conditions due to glycosylation.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.