TGFBR2 Protein, Human, Recombinant (ECD, His Tag)

TGFBR2 is a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. It is a transmembrane protein. TGFBR2 is comprised of a C-terminal protein kinase domain and an N-terminal ectodomain. The ectodomain consists of a compact fold containing nine beta-strands and a single helix stabilized by a network of six intra strand disulfide bonds. The folding topology includes a central five-stranded antiparallel beta-sheet, eight-residues long at its centre, covered by a second layer consisting of two segments of two-stranded antiparallel beta-sheets. TGFBR2 has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGFBR2 gene have been associated with Marfan syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. TGFBR2 attenuates the biological activities of TGF-beta in colorectal cancer. TGFBR2 expression is increased in oral squamous cell carcinoma cells. Its expression is decreased by IL-1beta while inducing Sp3 via NFkappaB. TGFB2 and TGFBR2 are involved in the antiestrogenic activity.

AAT3 Protein, Human; FAA3 Protein, Human; LDS1B Protein, Human; LDS2 Protein, Human; LDS2B Protein, Human; MFS2 Protein, Human; RIIC Protein, Human; TAAD2 Protein, Human; TGFbeta-RII Protein, Human; TGFR-2 Protein, Human

Measured by its binding ability in a functional ELISA. Immobilized TGFBR2h (1-166Q) (Cat:PH52429I2) at 10 μg/mL (100 μL/well) can bind TGFB1-His/Biotin (Cat:PH50635M2), the ECof human TGFB1-His/Biotin (Cat:PH50635M2) is 130-300 ng/mL.

Lyophilized from sterile 20 mM PBS, 150 mM NaCl, 10 % glycerol, 0.5 mM TCEP, pH 7.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant human TGFBR2 consists of 155 amino acids and predicts a molecular mass of 17.8 kDa. As a result of glycosylation, it migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.