MST1 Protein, Human, Recombinant (His Tag)

Product Specifications

Background

Dysregulation of MST1/STK4, a key kinase component of the Hippo-YAP pathway, is linked to the etiology of many cancers with poor prognosis. STK4/Hippo pathway may have important therapeutic implications for cancer. The tumor suppressor serine/threonine-protein kinase 4 (STK4) differentially regulates TLR3/4/9-mediated inflammatory responses in macrophages and thereby is protective against chronic inflammation-associated Hepatocellular carcinoma (HCC) . STK4 has potential as a diagnostic biomarker and therapeutic target for inflammation-induced HCC.

Overview

A DNA sequence encoding the human STK4 isoform 1 (Q13043-1) (Glu 2-Phe 487) was expressed, with a polyhistidine tag at the N-terminus.

Synonyms

KRS2 Protein, Human; MST1 Protein, Human; TIIAC Protein, Human; YSK3 Protein, Human

Gene Name

Serine/threonine kinase 4

UniProt

Q13043-1

Expression System

Baculovirus-Insect Cells

Tag

N-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by SDS-PAGE.

Activity

The specific activity was determined to be > 150 nmol/min/mg using MBP as substrate.

Form

Liquid

Buffer

Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins

Molecular Weight

The recombinant human STK4 consists of 505 amino acids and migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions as predicted.

Storage Conditions

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.