IL-6R Protein, Human, Recombinant (ECD, His & AVI Tag), Biotinylated

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.

A DNA sequence encoding the human IL6R (NP_000556.1) (Met1-Pro365) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

CD126 Protein, Human; gp80 Protein, Human; IL-6R Protein, Human; IL-6R-1 Protein, Human; IL-6RA Protein, Human; IL6Q Protein, Human; IL6RA Protein, Human; IL6RQ Protein, Human

Immobilized Human IL-6 (Cat:PH51185M2) at 2 μg/mL (100 μL/well) can bind Human IL-6R, Biotinylated (Cat:PH52282M2), the ECof Human IL-6R, Biotinylated (Cat:PH52282M2) is 20-160 ng/mL.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant human IL6R consists of 372 amino acids and predicts a molecular mass of 41.80 kDa. It migrates as an approximately 60.56 kDa band in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.