KIR2DL4 Protein, Human, Recombinant (hFc Tag)

Product Specifications

Background

KIR2DL4, also known as CD158d, is a member of the killer cell Ig-like receptor (KIR) family. KIRs are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous. KIR2DL4 is expressed in all NK cells and some T cells. KIR2DL4 activates the cytotoxicity of NK cells, despite the presence of an immunoreceptor tyrosine-based inhibition motif (ITIM) in its cytoplasmic tail. The ITIM was not necessary for activation of lysis by KIR2DL4. The activation signal of KIR2DL4 was sensitive to inhibition by another ITIM-containing receptor. The activation-deficient mutant of KIR2DL4 inhibited the signal delivered by the activating receptor CD16.

Overview

A DNA sequence encoding the human KIR2DL4 (ADY38409.1) (Met1-His242) was expressed with the Fc region of human IgG1 at the C-terminus.

Synonyms

CD158D Protein, Human; G9P Protein, Human; KIR Protein, Human; KIR-103AS Protein, Human; KIR-2DL4 Protein, Human; KIR103 Protein, Human; KIR103AS Protein, Human; KIR2DL4 Protein, Human

Gene Name

Killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4

Expression System

CHO Stable Cells

Tag

C-hFc

Field of Research

ITIM/ITAM Immunoreceptors and Related Molecules

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

CD Molecule proteins, Immune Checkpoint Proteins, Biological Target proteins

Molecular Weight

The recombinant human KIR2DL4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 462 amino acids and has a predicted molecular mass of 51.3 kDa. The apparent molecular mass of the protein is approximately 62, 34 and 32 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.