CHRNB3 Protein, Human, Recombinant (ECD, hFc Tag)
Product Specifications
Background
Cholinergic nicotinic receptor (CHRN) gene family has been known to mediate the highly additive effects of nicotine in the body, and implicated nicotine dependence (ND) and related phenotypes. Cigarette smoking is protective in Parkinson's disease (PD), possibly because of nicotine action on brain nicotinic-acetylcholine receptors. The beta3 nicotinic-acetylcholine receptor subunit (encoded by CHRNB3) is depleted in the striatum of PD patients and associated with nicotine dependence.
Gene Name
Cholinergic receptor, nicotinic, beta 3 (neuronal)
Expression System
HEK293 Cells
Tag
C-hFc
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 85 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant human CHRNB3 consists 446 amino acids and predicts a molecular mass of 51 kDa.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH52091M4.pdf
Symbol
CHRNB3
Species
Human
Protein ID
NP_000740.1
Overview
A DNA sequence encoding the human CHRNB3 (NP_000740.1) (Met1-Leu232) was expressed with the Fc region of human IgG1 at the C-terminus.
AA Sequence
Met1-Leu232
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_000740.1
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