NLGN4X Protein, Human, Recombinant (ECD, hFc Tag)

NLGN4X (Neuroligin 4 X-Linked) is a Protein Coding gene. This gene encodes a member of the type-B carboxylesterase/lipase protein family. The encoded protein belongs to a family of neuronal cell surface proteins. NLGN4X interacts with discs large homolog 4 (DLG4) . It is broadly expressed in the brain, ovary, and other tissues. Genetic mutations in NLGN4X, including point mutations and copy number variants (CNVs), have been associated with susceptibility to autism spectrum disorders (ASDs) . Pathogenic mutations in the NLGN4X gene in ASDs and/or mental retardation (MR) are rare. Based on in vitro models, NLGN4X knockdown directly impacts the neurodevelopmental process during the formation of neurons and their connections.

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.