What is the catalog number for NLGN4X Protein, Human, Recombinant (ECD, hFc Tag)?

The catalog number for NLGN4X Protein, Human, Recombinant (ECD, hFc Tag) is PH52089M4.

What sizes are available for NLGN4X Protein, Human, Recombinant (ECD, hFc Tag)?

NLGN4X Protein, Human, Recombinant (ECD, hFc Tag) is available in the following size(s): 100 µg.

Is NLGN4X Protein, Human, Recombinant (ECD, hFc Tag) in stock?

NLGN4X Protein, Human, Recombinant (ECD, hFc Tag) is currently made to order / not held in immediate stock. Contact us for lead time.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

NLGN4X Protein, Human, Recombinant (ECD, hFc Tag)

Product Specifications

Background

NLGN4X (Neuroligin 4 X-Linked) is a Protein Coding gene. This gene encodes a member of the type-B carboxylesterase/lipase protein family. The encoded protein belongs to a family of neuronal cell surface proteins. NLGN4X interacts with discs large homolog 4 (DLG4) . It is broadly expressed in the brain, ovary, and other tissues. Genetic mutations in NLGN4X, including point mutations and copy number variants (CNVs), have been associated with susceptibility to autism spectrum disorders (ASDs) . Pathogenic mutations in the NLGN4X gene in ASDs and/or mental retardation (MR) are rare. Based on in vitro models, NLGN4X knockdown directly impacts the neurodevelopmental process during the formation of neurons and their connections.

Overview

A DNA sequence encoding the human NLGN4X (NP_065793.1) (Met1-Ser676) was expressed with the Fc region of human IgG1 at the C-terminus.

Synonyms

ASPGX2 Protein, Human; AUTSX2 Protein, Human; HLNX Protein, Human; HNL4X Protein, Human; KIAA1260 Protein, Human; NLGN4 Protein, Human

Gene Name

Neuroligin 4, X-linked

Expression System

HEK293 Cells

Tag

C-hFc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant human NLGN4X consists 873 amino acids and predicts a molecular mass of 98.2 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.