IL17A & IL17F Heterodimer Protein, Human, Recombinant (His Tag)

Product Specifications

Overview

A DNA sequence encoding the human IL17A (NP_002181.1) (Met1-Ala155) and the mature form of human IL17F (NP_443104.1) (Arg31-Gln163) linked by a polypeptide linker was expressed with a polyhistidine tag at the C-terminus.

Synonyms

CTLA8 Protein, Human; IL-17 Protein, Human; IL-17A Protein, Human; IL17 Protein, Human

Gene Name

Interleukin 17A

Expression System

HEK293 Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

1.Measured by its binding ability in a functional ELISA. 2. Measured by its ability to induce IL-6 secretion by NIH 3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFα. The EDfor this effect is 0.8-4 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant human IL17A & IL17F consists 280 amino acids and predicts a molecular mass of 31 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.