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IL-23 Protein, Human, Recombinant (His Tag), Biotinylated

Product Specifications

Synonyms

IL-23 Protein, Human; IL-23A Protein, Human; IL23 Protein, Human; IL23P19 Protein, Human; P19 Protein, Human; SGRF Protein, Human

Expression System

HEK293 Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Biological Target proteins, Cytokine Proteins, Interleukin proteins, Biotinylated proteins

Molecular Weight

The recombinant human IL23 complex (IL23A&IL12B) consists 502 amino acids and predicts a molecular mass of 55.8 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Product Datasheet

http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH52052M2.pdf

Symbol

IL12B & IL23A

Species

Human

Protein ID

NP_002178.2&NP_057668.1

Overview

A DNA sequence encoding the human IL23 complex composed of IL12B (NP_002178.2) (Met1-Ser328) and the mature form of human IL23A (NP_057668.1) (Arg20-Pro189) linked by a polypeptide linker was fused with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.

AA Sequence

Met1-Ser328 & Arg20-Pro189

Protein ID Link

https://www.ncbi.nlm.nih.gov/protein/NP_002178.2&NP_057668.1

Available Sizes

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