CD16a Protein, Human, Recombinant (ECD, F176V, His & AVI Tag), Biotinylated

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.

A DNA sequence encoding the extracellular domain (Met1-Gln208) of human CD16a (P08637-1, with natural variant Phe 176 Val) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus (AVI-his) . The expressed protein was biotinylated in vivo by the Biotin–Protein ligase (BirA enzyme) which is co-expressed.

CD16 Protein, Human; CD16A Protein, Human; Fc gamma RIIIa Protein, Human; FCG3 Protein, Human; FCGR3 Protein, Human; FCGRIII Protein, Human; FCR-10 Protein, Human; FCRIII Protein, Human; FCRIIIA Protein, Human; IGFR3 Protein, Human; IMD20 Protein, Human

1. Measured by its ability to bind human IgG1 (Cat: PH52028M0) in functional ELISA. 2. Using the Octet RED System, the affinity constant (Kd) of CD16a Protein, Human, Recombinant (176 Val, His & AVI Tag), Biotinylated (Cat. PH52017M9) bound to Conbercept was 0.1 μM.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The secreted recombinant human CD16a consists of 227 amino acids and predicts a molecular mass of 25.6 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 48 KDa due to glycosylation.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.