CD32A Protein, Human, Recombinant (167 Arg, hFc Tag)
Product Specifications
Synonyms
CD32 Protein, Human; CD32A Protein, Human; CDw32 Protein, Human; Fc gamma RIIA Protein, Human; FCG2 Protein, Human; FcGR Protein, Human; FCGR2 Protein, Human; FCGR2A1 Protein, Human; IGFR2 Protein, Human
Gene Name
Fc fragment of IgG receptor IIa
Expression System
HEK293 Cells
Tag
C-hFc
Field of Research
Fc Receptors ITIM/ITAM Immunoreceptors and Related Molecules
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE.
Activity
Measured by its binding ability in a functional ELISA.Immobilized human CD32a-Fc (Cat:PH52002M4) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1 with a linear range of 0.625-10 μg/ml.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Fc receptor proteins, CD Molecule proteins, Receptor Proteins
Molecular Weight
The recombinant human CD32a/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 426 amino acids and predicts a molecular mass of 47.3 kDa. As a result of glycosylation, the apparent molecular mass of rh CD32a/Fc monomer is approximately 55 kDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH52002M4.pdf
Symbol
FCGR2A
Species
Human
Protein ID
AAA35827.1
Overview
A DNA sequence encoding the extracellular domain (Met 1-Ile 218) of human CD32a (AAA35827.1) was fused with the Fc region of human IgG1 at the C-terminus. It is identical to FCGR2A131H/R in the reference.
AA Sequence
Met1-Ile218
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/AAA35827.1
Available Sizes
Curated Selection
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