BTLA Protein, Human, Recombinant (hFc Tag)

Product Specifications

Background

BTLA is a inhibitory molecule which belongs to the Ig superfamily. It down-modulates immune responses. As such, reagents that regulate the binding of BTLA to its ligand or alter BTLA signaling have significant therapeutic promise. BTLA is crucial to understand the mechanism (s) of action of these antibodies before attempting clinical applications. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T (H) 1) but not T (H) 2 cells. BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1) .

Overview

A DNA sequence encoding the human BTLA (NP_001078826.1) (Met1-Thr134) was expressed with the Fc region of human IgG1 at the C-terminus.

Synonyms

BTLA1 Protein, Human; CD272 Protein, Human

Gene Name

B and T lymphocyte associated

Expression System

HEK293 Cells

Tag

C-hFc

Field of Research

Cancer Drug Targets ITIM/ITAM Immunoreceptors and Related Molecules

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

1.Measured by its binding ability in a functional ELISA. 2.Immobilized human BTLA-Fc (Cat:PH51996M4) at 10μg/mL (100μL/well) can bind biotinylated mouse HVEM-Fch (Cat:10567-M03S), the ECof biotinylated mouse HVEM-Fch is 6-70ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

B7 Family proteins, CD Molecule proteins, Cancer proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins

Molecular Weight

The recombinant human BTLA comprises 342 amino acids and has a predicted molecular mass 38.8 kDa. As a result of glycosylation, it migrates as an approximately 53.7 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.