NECTIN4/Nectin 4 Protein, Human, Recombinant (ECD, His Tag)

Product Specifications

Overview

A DNA sequence encoding the human NECTIN4 (NP_112178.2) (Met1-Ser349) was expressed with a polyhistidine tag at the C-terminus.

Synonyms

EDSS1 Protein, Human; LNIR Protein, Human; nectin-4 Protein, Human; PRR4 Protein, Human; PVRL4 Protein, Human

Expression System

HEK293 Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 85 % as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human NECTIN4 His (Cat: PH51950M2) at 2 μg/ml (100 μl/well) can bind Human PVRL1 His (Cat: PH50410M2), biotinylated, the ECof Human PVRL1 His, biotinylated is 200-800 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Biological Target proteins, Immune Checkpoint Proteins

Molecular Weight

The recombinant human NECTIN4 consists of 329 amino acids and predicts a molecular mass of 35.6 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.