Isocitrate dehydrogenase/IDH1 Protein, Human, Recombinant (His Tag)

Product Specifications

Overview

A DNA sequence encoding the human IDH1 (O75874) (Met 1-Leu 414) was fused with a polyhistidine tag at the C-terminus.

Synonyms

HEL-216 Protein, Human; HEL-S-26 Protein, Human; IDCD Protein, Human; IDH Protein, Human; IDP Protein, Human; IDPC Protein, Human; PICD Protein, Human

Gene Name

Isocitrate dehydrogenase 1 (NADP+), soluble

UniProt

O75874

Expression System

E. coli

Tag

C-His

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile 50mM Tris, 200mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins

Molecular Weight

The recombinant human IDH1 consisting of 424 amino acids and has a calculated molecular mass of 48 kDa. It migrates as an approximately 43 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.