Isocitrate dehydrogenase/IDH1 Protein, Human, Recombinant (His Tag)
Product Specifications
Synonyms
HEL-216 Protein, Human; HEL-S-26 Protein, Human; IDCD Protein, Human; IDH Protein, Human; IDP Protein, Human; IDPC Protein, Human; PICD Protein, Human
Gene Name
Isocitrate dehydrogenase 1 (NADP+), soluble
UniProt
O75874
Expression System
E. coli
Tag
C-His
Endotoxin
Please contact us for more information.
Purity
> 95 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile 50mM Tris, 200mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Enzyme Proteins
Molecular Weight
The recombinant human IDH1 consisting of 424 amino acids and has a calculated molecular mass of 48 kDa. It migrates as an approximately 43 kDa band in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH51937E2.pdf
Symbol
IDH1
Species
Human
Overview
A DNA sequence encoding the human IDH1 (O75874) (Met 1-Leu 414) was fused with a polyhistidine tag at the C-terminus.
Uniprot URL
https://www.uniprot.org/uniprot/O75874
AA Sequence
Met1-Leu414
Available Sizes
Curated Selection
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