TIE2 Protein, Human, Recombinant (ECD, His Tag)

TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

A DNA sequence encoding the extracellular domain (Met1-Lys745) of human Tie2 (AAA61139.1) precursor was fused with a polyhistidine tag at the C-terminus.

Measured by its binding ability in a functional ELISA. Immobilized recombinant human Tie2 at 2 μg/ml (100 μl/well) can bind human Angiopoietin-2 at a linear range of 1.28-160 ng/ml.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant human Tie2 comprises 734 amino acids and predicts a molecular mass of 82 kDa. As a result of glycosylation, the apparent molecular mass of rhTie2 is approximately 95-105 kDa in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.