EGFL6 Protein, Human, Recombinant (His Tag)

Product Specifications

Background

EGFL6, also known as EGF-L6, belongs to the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. EGFL6 contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. EGFL6 gene is expressed early during development, and its expression has been detected in lung and meningioma tumors.

Overview

A DNA sequence encoding the human EGFL6 (NP_056322.2) (Met1-Asp553) was expressed with a C-terminal polyhistidine tag.

Synonyms

MAEG Protein, Human; W80 Protein, Human

Gene Name

EGF-like-domain, multiple 6

Expression System

Baculovirus-Insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 60 % as determined by SDS-PAGE.

Activity

Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 x 10E4 cells/well areadded to EGFL6-His coated plates (5μg/mL and 100μL/well), approximately >40% will adhere specifically after 30 minutes at 37°C.

Form

Lyophilized

Buffer

Lyophilized from sterile 20mM PB, 500mM NaCl, 10% Glycerol, pH7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cytokine Proteins, Growth Factor proteins, Biological Target proteins

Molecular Weight

The secreted recombinant human EGFL6 consists of 543 amino acids and predicts a molecular mass of 60.5 KDa. The apparent molecular mass of the protein is approximately 62 KDa in SDS-PAGE under reducing conditions due to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.