HEXB Protein, Human, Recombinant (His Tag)
Product Specifications
Overview
A DNA sequence encoding the human HEXB (P07686) (Ala43-Met556) was expressed with a C-terminal polyhistidine tag.
Synonyms
ENC-1AS Protein, Human; HEL-248 Protein, Human
Gene Name
Hexosaminidase B (beta polypeptide)
UniProt
P07686
Expression System
HEK293 Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
Measured by its ability to hydrolyze 4methylumbelliferylNacetylβDglucosaminide (4MUGlcNAc) . The specific activity is > 3000 pmols/min/μg
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant human HEXB comprises 525 amino acids and has a predicted molecular mass of 60.2 kDa. The apparent molecular mass of the protein is approximately 60-66 kDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
HEXB
Species
Human
Uniprot URL
https://www.uniprot.org/uniprot/P07686
AA Sequence
Ala43-Met556
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