HEXB Protein, Human, Recombinant (His Tag)

Product Specifications

Overview

A DNA sequence encoding the human HEXB (P07686) (Ala43-Met556) was expressed with a C-terminal polyhistidine tag.

Synonyms

ENC-1AS Protein, Human; HEL-248 Protein, Human

Gene Name

Hexosaminidase B (beta polypeptide)

UniProt

P07686

Expression System

HEK293 Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its ability to hydrolyze 4methylumbelliferylNacetylβDglucosaminide (4MUGlcNAc) . The specific activity is > 3000 pmols/min/μg

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant human HEXB comprises 525 amino acids and has a predicted molecular mass of 60.2 kDa. The apparent molecular mass of the protein is approximately 60-66 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.