Caspase-3 Protein, Human, Recombinant (His Tag)

Product Specifications

Overview

A DNA sequence encoding the mature form of human caspase3 (Q5IS54) (Ser29-His277) was expressed with a polyhistidine tag at the C-terminus.

Synonyms

CPP32 Protein, Human; CPP32B Protein, Human; SCA-1 Protein, Human

Gene Name

Caspase 3, apoptosis-related cysteine peptidase

UniProt

Q5IS54

Expression System

E. coli

Tag

C-His

Field of Research

Cardiac Stem Cell Markers

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its ability to cleave the fluorogenic peptide substrate Ac-DEVD-AFC.The specific activity is > 5000 pmoles/min/μg.

Form

Lyophilized

Buffer

Lyophilized from sterile 20mM HEPES, 100mM NaCl, 1mM EDTA, 10% Sucrose, 0.1% chaps, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins, Biomarker Proteins

Molecular Weight

The recombinant human caspase3 consists of 259 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 17 and 15 KDa band in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.