IGFBP5 Protein, Human, Recombinant (His Tag)

Product Specifications

Overview

A DNA sequence encoding the human IGFBP5 (NP_000590.1) (Met1-Glu272) was expressed with a polyhistidine tag at the C-terminus.

Synonyms

IBP5 Protein, Human

Gene Name

Insulin-like growth factor binding protein 5

Expression System

Baculovirus-Insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. The EDfor this effect is 0.1-0.5 μg/mL in the presence of 14 ng/mL rhIGF-II.

Form

Lyophilized

Buffer

Lyophilized from sterile 20 mM Tris, pH 8.0, 300 mM NaCl, 10 % glycerol. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Growth Factor proteins

Molecular Weight

The recombinant human IGFBP5 consists of 263 amino acids and predicts a molecular mass of 30 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.