VEGF165 Protein, Human, Recombinant (His & AVI Tag), Biotinylated
Product Specifications
Background
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF) /vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.
Synonyms
MVCD1 Protein, Human; VEGF Protein, Human; VEGF165 Protein, Human; VPF Protein, Human
Gene Name
Vascular endothelial growth factor A
Expression System
HEK293 Cells
Tag
C-AVI & His
Field of Research
Cancer Drug Targets
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) . The EDfor this effect is typically 4-16 ng/mL.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Cytokine Proteins, Biological Target proteins, Diagnostic proteins, Biomarker Proteins, Biotinylated proteins, Growth Factor proteins, Cancer proteins, Immunotherapy proteins, Cell Therapy proteins
Molecular Weight
The secreted recombinant human VEGF165 consists of 200 amino acids and predicts a molecular mass of 23.1 kDa. As a result of glycosylation, it migrates as an approximately 28.3 kDa band in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH51760M9.pdf
Symbol
VEGFA
Species
Human
Protein ID
P15692-4
Overview
A DNA sequence encoding the human VEGF165 (P15692-4) (Ala27-Arg191) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
AA Sequence
Ala27-Arg191
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/P15692-4
Available Sizes
Curated Selection
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