FANCA Protein, Human, Recombinant (His Tag)

Product Specifications

Background

FANCA is one of the six known Fanconi anemia gene products (FANCA, FANCC, FANCD2, FANCE, FANCF, and FANCG proteins) . Fanconi anemia (FA) is a genetic disorder predisposing to aplastic anemia and cancer characterized by hypersensitivity to DNA-damaging agents and oxidative stress. FANCA associates with the IκB kinase (IKK) signalsome via interaction with IKK2. Components of the FANCA complex undergo rapid, stimulus-dependent changes in phosphorylation, which are blocked by kinase-inactive IKK2.

Overview

A DNA sequence encoding the human FANCA (AAH08979.1) (Met 1-Cys 297) was expressed, with a polyhistidine tag at the N-terminus.

Synonyms

FA Protein, Human; FA-H Protein, Human; FA1 Protein, Human; FAA Protein, Human; FACA Protein, Human; FAH Protein, Human; FANCH Protein, Human

Gene Name

Fanconi anemia, complementation group A

Expression System

Baculovirus-Insect Cells

Tag

N-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 75 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile 20mM Tris, 200mM NaCl, pH 8.5, 20% glycerol, 0.1% Triton, 1mM TCEP Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant human FANCA consists of 316 amino acids and predicts a molecular mass of 35.2 kDa. It migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.