LAG3 Protein, Human, Recombinant (ECD, hFc Tag)

Product Specifications

Background

LAG3 (Lymphocyte Activating 3) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. LAG3, also known as CD223 and Lymphocyte activation gene 3, belongs to the immunoglobulin (Ig) superfamily. The LAG3 gene contains 8 exons. It is selectively expressed in activated T and NK cells. LAG3 contains 4 extracellular Ig-like domains and has a negative regulatory function in T cells. It also acts as a new marker of T cell-induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signaling, leading to increased antigen-specific T-cell responses in vivo. Diseases associated with LAG3 include Smoldering Myeloma and Kyphoscoliotic Heart Disease.

Overview

A DNA sequence encoding the human LAG3 (NP_002277.4) (Met1-Leu450) was expressed with the Fc region of human IgG1 at the C-terminus.

Synonyms

CD223 Protein, Human; LAG-3 Protein, Human

Gene Name

Lymphocyte-activation gene 3

Expression System

HEK293 Cells

Tag

C-hFc

Field of Research

Cancer Drug Targets

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Immobilized LAG3 Protein, Human, Recombinant (ECD, hFc Tag) (Cat:PH51594M4) at 2 μg/mL (100 μL/well) can bind Anti-LAG3 (BMS) -IgG4 Antibody (Relatlimab) (Cat:68075-H001), the ECis 8-40 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile 20mM NaH2PO4, 150mM NaCl, 0.1M Arg, 0.1M Glu, 10% glycerol, 0.01% tween20, pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Immune Checkpoint Proteins, Biological Target proteins, CD Molecule proteins, Cancer proteins, Receptor Proteins, Immunotherapy proteins

Molecular Weight

The recombinant human LAG3 consists of 666 amino acids and predicts a molecular mass of 72.9 kDa. As a result of glycosylation, it migrates as an approximately 84.19 KDa band in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.