VMO1 Protein, Human, Recombinant (mFc Tag)
Product Specifications
Background
Vitelline membrane outer layer protein 1 (VMO1), a basic protein present in the outer layer of the vitelline membrane of eggs, plays essential roles in separating the yolk from the egg white and preventing infection from bacteria by forming a barrier of fibrous layers in avian eggs. VMO1 is regulated by estrogen and target microRNAs and it is a potential diagnostic marker of ovarian cancer in laying hens.
Synonyms
ERGA6350 Protein, Human; PRO21055 Protein, Human
Gene Name
Vitelline membrane outer layer 1 homolog (chicken)
Expression System
HEK293 Cells
Tag
C-mFc
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 80 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant human VMO1 consists 412 amino acids and predicts a molecular mass of 45.4 kDa.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH51582M4.pdf
Symbol
VMO1
Species
Human
Protein ID
NP_872372.1
Overview
A DNA sequence encoding the human VMO1 (NP_872372.1) (Met1-Ser202) was expressed with the Fc region of mouse IgG1 at the C-terminus.
AA Sequence
Met1-Ser202
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_872372.1
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