DCLK1 Protein, Human, Recombinant

Product Specifications

Background

DCAMKL1, also known as DCLK1, is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Its microtubule-polymerizing activity is independent of its protein kinase activity. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.

Overview

A DNA sequence encoding the human DCLK1 (O15075-1) (Met 1-Val 705) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

Synonyms

CL1 Protein, Human; CLICK1 Protein, Human; DCAMKL1 Protein, Human; DCDC3A Protein, Human; DCLK Protein, Human

Gene Name

Doublecortin-like kinase 1

UniProt

O15075-1

Expression System

Baculovirus-Insect Cells

Tag

No tag

Field of Research

Calcium-binding Proteins and Related Molecules

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by SDS-PAGE.

Activity

The specific activity was determined to be > 3.5 nmol/min/mg using synthetic Autocamtide-2 peptide (KKALRRQETVDAL-amide) as substrate.

Form

Liquid

Buffer

Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

Function

Kinase proteins, Enzyme Proteins

Molecular Weight

The secreted recombinant human DCLK1 consists of 707 amino acids and predicts a molecular mass of 78.5 KDa. The apparent molecular mass of the protein is approximately 64 KDa in SDS-PAGE under reducing conditions due to glycosylation.

Storage Conditions

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.