BMPRIB Protein, Human, Recombinant (aa 149-502, His & GST Tag)

Product Specifications

Background

BMPR1B (bone morphogenetic protein receptor, type IB), also known as ALK6, is a member of the bone morphogenetic protein (BMP) receptor family. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. BMPR1B is the major transducer of signals in precartilaginous condensations as demonstrated in experiments using constitutively active BMPR1B receptors. BMPR1B is a more effective transducer of GDF5 than BMPR1A. Unlike BMPR1A null mice, which die at an early embryonic stage, BMPR1B null mice are viable.

Overview

A DNA sequence encoding the human ALK6 (NP_001194.1) cytoplasmic domain (Arg 149-Leu 502) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Synonyms

ALK-6 Protein, Human; ALK6 Protein, Human; CDw293 Protein, Human

Gene Name

Bone morphogenetic protein receptor, type IB

Expression System

Baculovirus-Insect Cells

Tag

N-GST & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Kinase activity untested

Form

Liquid

Buffer

Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.5, 20% glycerol, 0.3mM DTT Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

Function

Kinase proteins, TGF beta Family proteins, Receptor Proteins, Enzyme Proteins, Cytokine Proteins

Molecular Weight

The recombinant human ALK6 (149-502) /GST chimera consists of 591 amino acids and has a calculated molecular mass of 68.3 KDa. It migrates as an approximately 55 KDa band in SDS-PAGE under reducing conditions.

Storage Conditions

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.